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High Performance Liquid Chromatography System
Overall Design Description
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DAC150 HPLC Overall Design Description | ||
SN. | Name | Technical Index |
1 | System composition | Dynamic axial compression column, infusion pump/UV detector, software, auxiliary equipment, etc. |
2 | System flow rate | 1000ml/min |
3 | System pressure | 10MPa |
4 | Ambient temperature requirement | 5℃ ~60℃ |
5 | Column Specifications | DAC150*650 |
6 | Detection wavelength | 190-400nm |
7 | FAD Compliance | FDA 21 CFR Part 11 |
8 | Material | All materials contact with liquid meet requirements of hygienic grade. Pumps, pipes, seals, etc. are free of leakage and rust. Material: Stainless steel 316L. Hose and sealing gasket are made of food silicone rubber, which meet requirements of FDA, USP Class VI or European Pharmacopoeia, and the material proof document will be provided in the verification document. |
Equipment Description
DAC150 Dynamic axial compression column
Dynamic axial compression technology completes column loading, column pressure maintenance and column unloading work through the movement of piston.
The periphery of piston is equipped with specially designed sealing structure that allows piston to slide freely along inner wall of column tube while maintaining high seal.
Piston movement and pressure maintenance rely on pressure holding system, hydraulic power is more stable and uniform than traditional axial compression column using spring power, which makes the use effect of separation column better. It can be loaded of diameters (150mm-1000mm) Column while maintaining the same separation effect as the analytical column.
DAC150 Dynamic Axial Compression Column | ||
Sn. | Name | Technical Index |
1 | Column tube diameters and length | Inner diameter: ID150mm Length: 1000mm |
2 | Designed pressure | 10MPa |
3 | Column tube material, finish | Material :316L Inner roughness Ra≤0.4 μm External surface roughness Ra<1.6 μm |
4 | Sieve plate aperture and material | Aperture: 2um Material: 316L |
5 | Piston and Column Bottom Distribution | Forced distribution Material 316L Distribution groove surface finish: 0.4 |
6 | Liquid collect material | SS316L/PTFE |
7 | Others | Column bottom quick mounting connection |
(1) Hydraulic cylinder
Piston adopts two sets of V-shaped combined seals and ceramic strong support sleeve.
The static seal of piston adopts 4 O-rings, the piston does not rotate, and lock nut is directly locked, which ensures that the seal does not rotate.
End face of the live cold rod has a quick start opening groove.
The guide sleeve adopts V-shaped combined seals for the front and rear shafts. The aluminum oil-free bearing is added to the guide sleeve. The end cover is installed by screws to ensure that the seal is not twisted and the life is guaranteed. This product is designed with 5-year service life warranty and maintenance-free.
(2) Sieve plate sealing structure
Sealing structure of piston sieve plate directly affects performance of column efficiency and separation degree. Sieve plate installation and sealing is not good, will lead to in the process of column loading, the filler in the homogenate will recoil from the sieve plate and sealing joint to the distribution name, which is firmly not allowed to appear; Moreover, due to the large diameter, unreasonable structural design, there will be peripheral compaction, compaction in the center of the phenomenon.
The sieve plate seal adopts the sealing structure of the sieve plate and tetrafluoron cold pressure interference installation, the sieve plate and tetrafluoron as one, good sealing performance, convenient loading and unloading of the sieve plate, the diameter of the sieve plate is infinitely close to the diameter of the column tube, and the final separation is good, the column efficiency is high, and the tail is small.
(3) High pressure sealing ring
The high-pressure sealing ring adopts imported spring energy storage sealing ring. The sealing ring is a pressure-assisted sealing device with a PTFE jacket, which is specially equipped with a corrosion-resistant metal accumulator spring. The spring is compressed, urging the lip of the jacket against the sealing groove, thereby
forming a seal.
(4) Reversing ball valve (up, down)
Choose Pister valve (4KH-, its high pressure valve is the world's leading and innovative fluid technology manufacturer, its products are widely used in various industrial fields. The main products include: various ball valves, globe valves, high pressure control valves, integrated valves, solenoids Valves and Combination Quick Changers.
(5) Others
FESTO is used for air components, and valves imported from Germany are used for oil components, which improves the sealing performance of the entire system, has no leakage, has high reliability, and increases the service life of the product.
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Regulators LR/LRS/LRB/LRBS, D series | Filters LF/LFMA/LFMB/LFX/LFMBA, D series |
1000ml High pressure pump
The high-pressure preparation pump is a high-precision infusion pump system operated by PLC control touch screen. Based on the all-digital frequency generation system, advanced motor drive system and friendly man-machine display interface, the operation and control of the equipment are very convenient. The advanced pump head and one-way valve design ideas integrate the most advanced experience in the world today, so that users can ensure the accuracy and repeatability of infusion under various conditions of use.
100ml High Pressure Pump | ||
Sn. | Name | Technical Index |
1 | Flowrate range | 0.01-999.99ml/min |
2 | Maximum pressure | 10MPA |
3 | Flow accuracy | ± 0.5% |
4 | Flow repeatability | RSD≤ 0.3% |
5 | pressure pulsation | ≤ 0.3MPA |
6 | Tube connection | 1/16"OD, 0.75mmID |
7 | Pump head material | Stainless Steel 316L, PEEK |
UV detector
UV/Visible wavelength detectors are an important part of a complete HPLC purification system. The detector design is guided by today's state-of-the-art technology, and its baseline noise and drift are reduced to a new limit through digital data processing and control. Due to the digital output function, the detector can be directly connected with the computer through the serial port without any data acquisition unit.
The detector system adopts different flow cell unit designs for different flow rates, which fully meets the requirements of direct detection without shunt, which is a very prominent shining point of the device.
UV Detector | ||
SN. | Name | Technical Index |
| 1 | Maximum pressure | 10MPa |
| 2 | Flowrate range | 0-1000ml |
| 3 | wavelength coverage | 190-400nm |
| 4 | wavelength accuracy | ± 1nm |
| 5 | Wavelength reproducibility | 0.2nm |
| 6 | detection range | 0-3AU |
| 7 | baseline noise | 1× 10^-5 AU/h |
| 8 | baseline drift | 1.5× 10^-4AU/h |
| 9 | time constant | 16 grade (adjustable) |
| 10 | Material | Stainless steel/quartz /PFA/PEEK |
| 11 | tube connection | 1/8" PEEK or 316L stainless steel |
ID100 online filter
ID30 Online filter | ||
SN. | Name | Technical Index |
1 | Diameter | Inner size: ID100mm |
2 | Design pressure | 10MPa |
3 | Sieve plate aperture and material | Aperture: 3um |
4 | Material | 316L |
Dynamic mixer
The main function of this instrument is to mix two different solutions uniformly in binary gradient test, and the ideal state is to minimize the mixing volume. The motor speed is 500rpm/min, which can ensure the uniform mixing of the solution.
The middle layer of the mixing chamber is composed of upper and lower halves. Through the rotation of the stirring bar, the liquid in the lower half can be rapidly and intensively mixed, which is dynamic. The upper part is tightly wrapped with glass wool to achieve static solution mixing service
Dynamic Mixer | ||
Sn. | Name | Technical Index |
1 | Maximum pressure | 10MPa |
2 | Flow range | 0-1000ml |
3 | Volume | 6ml |
4 | Material | 316L |
Chromatographic data processing workstation software
1. LabChrom complies with GLP/FDA-21CFR PartII Electronic Signature Certification, Data Validation Security, System Qualification Tool (IQ/OQ) and System Suitability Test (SST)
2. LabChrom is software product used to control and detect chromatographic systems. It consists of control software and spectral analysis and processing software.
It can be quickly connected to the chromatography system, accurate acquisition and has the function of fast and accurate analysis and calibration. Modular design makes it easier and faster to complete each operation and operation while the operation is simple.
SN. | Name | Technical Index |
1 | Basic parameter settings and acquisition functions | The system can monitor various parameters during the operation of the entire purification system online, and can display the set and actual parameter values at the same time; such as time, flow, sample peaking, etc., and can set important parameters, If some parameters are out of the setting range, the system can make relevant response (alarm or automatically stop running) |
2 | Record backup function | Record the data in the whole production process (such as chromatogram, etc.) and be able to print and save the map. All production data should be able to be backed up and restored, and the data can be stored in a USB flash drive or other mobile storage devices, or there are relevant measures to ensure that the computer appears. Production data can be effectively recovered after an exception. |
3 | calculation function | Calculation of chromatographic column performance, such as the calculation of the theoretical plate number, resolution, symmetry coefficient and other data of chromatographic peaks |
4 | Warning alarm function | The control software divides the operating state of the system into three levels, namely normal work, warning and alarm, and has corresponding color and sound prompts, so that the operator can better understand the working state of the entire system according to the system feedback information |
5 | Permission function | All operators can set different permissions, including equipment operation permissions, data access permissions, etc. |
6 | Check Tracking | The entire system has detailed historical records, archiving, inspection, and tracking from the user login to the completion of purification. Once a problem occurs, it can be traced back to the entire process. |
3. LabChrom User Interface introduction
Project Management (PM)
Sample management
All chromatograms saved and collected are stored under sample management. It will be easy to find and preview. Double-click to open any selected chromatogram, the software will automatically calculate the chromatogram processing results according to the analysis method. The analysis module includes: display and comparison of the chromatogram, automatic data integration, fast manual integration operation, and peak result information display.
Chromatogram Collection-Instrument operation method and data collection
Through the collection interface of the chromatogram, realizing the data collection, equipment situation, method display, and realizing the procedure start-up, stop, pause and continue operation etc
Supplying List
Sn. | Name | Qty |
1 | DAC150 Dynamic axial compression column | 1 set |
2 | High pressure infusion pump (1000ml/min) | 2 pcs |
3 | UV Detector | 1 pc |
4 | ID100 online filter | 1 set |
5 | Dynamic mixer | 1 set |
6 | Chromatography data processing workstation software | 1 set |
7 | Preparation flow tank | 1 set |
8 | Manually prepare the injection valve | 1 set |
9 | 20ml loop | 1 set |
10 | Lab computer | 1 set |
11 | System startup tools and accessories kits | 1 set |
12 | Related documents (instructions) | 1 set |
13 | solvent tray | 1 set |
Tools and accessories List
Sn. | Name | QTY |
1 | ID100 Column bottom sieve plate | 1 pc |
2 | ID100 piston sieve plate | 1 pc |
3 | ID100 Column bottom sieve plate sealing ring | 2 pcs |
4 | ID100 piston sieve plate sealing ring | 2 pcs |
5 | Filter sieve plate | 1 pc |
6 | Filter sealing ring | 2 pcs |
7 | Column joint | 6 pcs |
8 | Protective tube | 6 pcs |
9 | PEEK capillary tube | 4 meters |
10 | PTFE pipe | 4 meters |
11 | Stainless steel joint | 2 sets |
12 | Stainless steel joint | 2 sets |
13 | Stainless steel joint | 2 sets |
14 | M4 inner hexagon screw | 6 pcs |
| 15 | Pipette 2meters | 4 pcs |
| 16 | Pipette nuts | 4 pcs |
| 17 | Pipette nuts | 4 pcs |
| 18 | Pipette dipsey/sinker | 4 pcs |
| 19 | Wiring board | 2 sets |
| 20 | Power line | 6 pcs |
| 21 | Junk ring | 10 pcs |
22 | Hollow screw | 4 pcs |
23 | Extended hollow screw | 4 pcs |
24 | Double-end Open spanner | 2 pcs |
25 | Double-end open spanner | 2 pcs |
26 | internal hexagonal wrench | 2 pcs |
27 | clyburn spanner 375 | 2 pcs |
| 28 | cross screwdriver | 2 pcs |
| 29 | Injection valve bracket | 2 pcs |
| 30 | M3*8 pan head screws | 4 sets |
| 31 | COM connecting line | 6 pcs |
| 32 | USB-COM convertor | 2 sets |
| 33 | Pump exhaust tool | 2 sets |
